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Entry M0096    3.5.3.3    creatinase

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Step 03

His232 deprotonates the hydroxyl group added, eliminating sarcosine.

GIF of Reaction Step M0096.stg03



Mechanisms

Bimolecular Elimination

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Collapse
Intermediate Terminated
Overall Product Formed

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Glu358 Side Chain spectator Hydrogen Bond Acceptor
His232 Side Chain reactant Proton Acceptor
Hydrogen Bond Acceptor
Glu262 Side Chain spectator Hydrogen Bond Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
C-N
O-H
The C-O bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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