spacer

Overview for MACiE Entry M0096

Version history

General Information

EC Number: 3.5.3.3 (A member of the Hydrolases, Acting on carbon-nitrogen bonds, other than peptide bonds, In linear amidines)

Enzyme Name: creatinase

Biological Species: Pseudomonas putida (Bacteria)

Catalytic Chain UniprotKB Accession Codes:

Representative PDB Code: 1chm - ENZYMATIC MECHANISM OF CREATINE AMIDINOHYDROLASE AS DEDUCEDFROM CRYSTAL STRUCTURES (Resolution = 1.90 Å).

Catalytic CATH Codes:

  • 3.90.230.10 - Creatinase/methionine aminopeptidase superfamily

"Other" CATH Codes:

  • 3.40.350.10 - Creatine Amidinohydrolase, subunit A, domain 1

Display structure information

Overall Reaction:

Image of creatine

Image of water

right arrow

Image of proton

Image of urea

Image of sarcosine

creatine
C00300
CHEBI:57947
water
C00001
CHEBI:15377
proton
C00080
CHEBI:24636
urea
C00086
CHEBI:16199
sarcosine
C00213
CHEBI:15611

View similar reactions


Stepwise Description of the Reaction

Step 1His232 deprotonates water, which initiates a nucleophilic attack on the guanidinium carbon of the substrate in an addition reaction.
Step 2The methylated nitrogen of the guanidinium group deprotonates His232.
Step 3His232 deprotonates the hydroxyl group added, eliminating sarcosine.
Step 4water deprotonates His232 in an inferred return step.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
His 232 A Side Chain
Glu 262 A Side Chain
Glu 358 A Side Chain

References

  1. W. T. Lowther et al. (1999), Biochemistry, 38, 7678-7688. Escherichia coli methionine aminopeptidase: implications of crystallographic analyses of the native, mutant, and inhibited enzymes for the mechanism of catalysis.
    Medline: 10387007
  2. M. Coll et al. (1990), J. Mol. Biol., 214, 597-610. Enzymatic mechanism of creatine amidinohydrolase as deduced from crystal structures.
    Medline: 1696320

Homologue information for M0096 (1chm)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

GOA logo
()
()
()
spacer
spacer