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Overview for MACiE Entry M0094

Version history

General Information

EC Number: 3.1.1.47 (A member of the Hydrolases, Acting on ester bonds, Carboxylic-ester hydrolases)

Enzyme Name: 1-alkyl-2-acetylglycerophosphocholine esterase

Biological Species: Bos taurus (Cow)

Catalytic Chain UniprotKB Accession Codes:

  • Q29460 - Platelet-activating factor acetylhydrolase IB subunit gamma

Representative PDB Code: 1bwp - PROBING THE SUBSTRATE SPECIFICITY OF THE INTRACELLULARBRAIN PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE (Resolution = 2.10 Å).

Catalytic CATH Codes:

Display structure information

Overall Reaction:

Image of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine

Image of water

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Image of 1-organyl-2-lyso-sn-glycero-3-phosphocholine

Image of acetate

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine
C04598
CHEBI:58666
water
C00001
CHEBI:15377
1-organyl-2-lyso-sn-glycero-3-phosphocholine
C04317
CHEBI:55397
acetate
C00033
CHEBI:30089

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Stepwise Description of the Reaction

Step 1His195 deprotonates Ser47.
Step 2Ser47 initiates a nucleophilic attack upon the carbonyl carbon of the substrate in an addition reaction.
Step 3The oxyanion formed collapses, eliminating phosphocholine with concomitant deprotonation of His195.
Step 4His195 deprotonates water, which initiates a nucleophilic attack upon the carbonyl carbon of the acyl-intermediate in an addition reaction.
Step 5The oxyanion collapses, eliminating Ser47 with concomitant deprotonation of His195.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Ser 47 A Side Chain
Gly 74 A Main Chain Amide
Asn 104 A Side Chain
Asp 192 A Side Chain
His 195 A Side Chain

References

  1. Y. S. Ho et al. (1999), Protein Eng., 12, 693-700. Probing the substrate specificity of the intracellular brain platelet-activating factor acetylhydrolase.
    Medline: 10469831
  2. Y. S. Ho et al. (1997), Nature, 385, 89-93. Brain acetylhydrolase that inactivates platelet-activating factor is a G-protein-like trimer.
    Medline: 8985254

Homologue information for M0094 (1bwp)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

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1-alkyl-2-acetylglycerophosphocholine esterase activity (molecular function)
protein binding (molecular function)
cytoplasm (cellular component)
spermatogenesis (biological process)
lipid catabolic process (biological process)
hydrolase activity (molecular function)
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