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Entry M0092    1.1.1.22    UDP-glucose 6-dehydrogenase

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Step 05

The oxyanion collapses, eliminating Cys260.

GIF of Reaction Step M0092.stg05



Mechanisms

Unimolecular Elimination by the Conjugate Base

Mechanism Components

Overall Product Formed
Enzyme-Substrate Bond Cleavage
Bond Cleavage
Bond Order Change
Intermediate Collapse
Intermediate Terminated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp264 Side Chain spectator Hydrogen Bond Donor
Lys204 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Thr118 Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Asn208 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Glu145 Side Chain spectator Not Active
Cys260 Side Chain reactant Nucleofuge

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
None
C-S
The C-O bond changes from a single to double bond
C
O
S

View similar reactions in MACiE.


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