spacer

Overview for MACiE Entry M0091

Version history

General Information

EC Number: 2.4.2.2 (A member of the Transferases, Glycosyltransferases, Pentosyltransferases)

Enzyme Name: pyrimidine-nucleoside phosphorylase

Biological Species: Bacillus stearothermophilus (Bacteria)

Catalytic Chain UniprotKB Accession Codes:

  • P77836 - Pyrimidine-nucleoside phosphorylase

Representative PDB Code: 1brw - THE CRYSTAL STRUCTURE OF PYRIMIDINE NUCLEOSIDEPHOSPHORYLASE IN A CLOSED CONFORMATION (Resolution = 2.10 Å).

Catalytic CATH Codes:

  • 3.40.1030.10 - Pyrimidine Nucleoside Phosphorylase; Chain A, domain 2

"Other" CATH Codes:

  • 1.20.970.10 - Transferase, Pyrimidine Nucleoside Phosphorylase; Chain C
  • 3.90.1170.30 - Aldehyde Oxidoreductase; domain 3

Display structure information

Overall Reaction:

Image of uridine

Image of phosphate

right arrow

Image of uracil

Image of alpha-D-ribose 1-phosphate

uridine
C00299
CHEBI:16704
phosphate
C00009
CHEBI:18367
uracil
C00106
CHEBI:17568
alpha-D-ribose 1-phosphate
C00620
CHEBI:57720

View similar reactions


Stepwise Description of the Reaction

Step 1His82 deprotonates phosphate.
Step 2In a heterolysis reaction, the C-N bond between ribose and the purine is cleaved.
Step 3Phosphate initiates a nucleophilic attack on the ribose in an addition reaction.
Step 4The mucleoside deprotonates His86.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
His 82 A Side Chain
Arg 168 A Side Chain
Ser 183 A Side Chain
Lys 187 A Side Chain

References

  1. M. J. Pugmire et al. (1998), Structure, 6, 1467-1479. The crystal structure of pyrimidine nucleoside phosphorylase in a closed conformation.
    Medline: 9817849

Homologue information for M0091 (1brw)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

GOA logo
pyrimidine nucleobase metabolic process (biological process)
pyrimidine nucleoside metabolic process (biological process)
metabolic process (biological process)
pyrimidine-nucleoside phosphorylase activity (molecular function)
transferase activity (molecular function)
transferase activity, transferring glycosyl groups (molecular function)
transferase activity, transferring pentosyl groups (molecular function)
metal ion binding (molecular function)
spacer
spacer