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Entry M0089    4.2.3.7    pentalenene synthase

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Step 06

His309 deprotonates the carbon adjacent to the new carbocation, forming the final double bond of pentalenene.

GIF of Reaction Step M0089.stg06



Mechanisms

Proton Transfer

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Overall Product Formed
Intermediate Terminated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His309 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Phe76 Side Chain spectator Electrostatic Stabiliser
van der Waals interaction
Phe77 Side Chain spectator Electrostatic Stabiliser
van der Waals interaction
Asn219 Side Chain spectator Electrostatic Stabiliser
Polar Non-polar interaction
Trp308 Side Chain spectator Electrostatic Stabiliser
van der Waals interaction

Metal Cofactors involved in Step 06

Metal Type Metal Identity Chain Activity Function
magnesium MG(not in PDB) 1 x spectator Substrate Binding

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
C-H
The C-C bond changes from a single to double bond
C
H
N

View similar reactions in MACiE.


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