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Overview for MACiE Entry M0079

Version history

General Information

EC Number: 2.4.2.21 (A member of the Transferases, Glycosyltransferases, Pentosyltransferases)

Enzyme Name: nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase

Biological Species: Salmonella typhimurium (Bacteria)

Catalytic Chain UniprotKB Accession Codes:

  • Q05603 - Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase

Representative PDB Code: 1d0s - CRYSTAL STRUCTURE OF NICOTINATE MONONUCLEOTIDE : 5,6-DIMETHYLBENZIMIDAZOLE PHOSPHORIBOSYLTRANSFERASE (COBT) FROMSALMONELLA TYPHIMURIUM COMPLEXED WITH 5, 6-DIMETHYLBENZIMIDAZOLE (Resolution = 1.90 Å).

Catalytic CATH Codes:

"Other" CATH Codes:

  • 1.10.1610.10 - 5,6-Dimethylbenzimidazole Phosphoribosyltransferase; Chain: A; domain 1

Display structure information

Overall Reaction:

Image of nicotinate D-ribonucleotide

Image of dimethylbenzimidazole

right arrow

Image of nicotinate

Image of N1-(5-phospho-alpha-D-ribosyl)-5,6-dimethylbenzimidazole

nicotinate D-ribonucleotide
C01185
CHEBI:57502
dimethylbenzimidazole
C03114
CHEBI:15890
nicotinate
C00253
CHEBI:32544
N1-(5-phospho-alpha-D-ribosyl)-5,6-dimethylbenzimidazole
C04778
CHEBI:57918

Overall Comment: This enzyme [CobT] is one of the enzymes involved in adenosylcobalamin formation.


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Stepwise Description of the Reaction

Step 1The N1 of dimethylbenzimidazole initiates a nucleophilic attack on the C1 of the nicotinate D-ribonucleotide in a substitution reaction, eliminating nicotinate.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Glu 317 A Side Chain

References

  1. C. G. Cheong et al. (1999), Biochemistry, 38, 16125-16135. The three-dimensional structures of nicotinate mononucleotide:5,6- dimethylbenzimidazole phosphoribosyltransferase (CobT) from Salmonella typhimurium complexed with 5,6-dimethybenzimidazole and its reaction products determined to 1.9 A resolution.
    Medline: 10587435

Homologue information for M0079 (1d0s)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ

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nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity (molecular function)
nucleoside biosynthetic process (biological process)
cobalamin biosynthetic process (biological process)
transferase activity (molecular function)
transferase activity, transferring glycosyl groups (molecular function)
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