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Entry M0078    2.3.3.1    citrate (Si)-synthase

Next Step

Step 01

Asp375 deprotonates the CH3 of acetyl-CoA, with concomitant double bond rearrangement.

GIF of Reaction Step M0078.stg01



Mechanisms

Proton Transfer
Assisted Keto-Enol Tautomerisation

Mechanism Components

Overall Reactant Used
Bond Cleavage
Bond Formation
Bond Order Change
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Ser244 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
His274 Side Chain spectator Hydrogen Bond Acceptor
Hydrogen Bond Donor
Asp375 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
His320 Side Chain spectator Hydrogen Bond Donor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
C-H
The C-C bond changes from a single to double bond
The C-O bond changes from a double to single bond
C
H
O

View similar reactions in MACiE.


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