Overview for MACiE Entry M0078
EC Number: 188.8.131.52 (A member of the Transferases, Acyltransferases, Acyl groups converted into alkyl groups on transfer)
Enzyme Name: citrate (Si)-synthase
Biological Species: Gallus gallus (Chicken)
Catalytic Chain UniprotKB Accession Codes:
- P23007 - Citrate synthase, mitochondrial
Representative PDB Code: 1al6 - CHICKEN CITRATE SYNTHASE COMPLEX WITH N-HYDROXYAMIDO-COA ANDOXALOACETATE (Resolution = 1.85 Å).
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Stepwise Description of the Reaction
|Step 1||Asp375 deprotonates the CH3 of acetyl-CoA, with concomitant double bond rearrangement.|
|Step 2||The oxyanion collapses, initiating a nucleophilic attack on the carbonyl carbon of oxaloacetate in an addition reaction with concomitant deprotonation of His320.|
|Step 3||His320 deprotonates water, initiating a nucleophilic attack on the carbonyl carbon of the CoA intermediate in a substitution reaction that eliminates CoA with concomitant deprotonation of Asp375.|
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Catalytic Residues Involved
||Location of Function
- M. Karpusas et al. (1990), Biochemistry, 29, 2213-2219. Proposed mechanism for the condensation reaction of citrate synthase: 1.9-A structure of the ternary complex with oxaloacetate and carboxymethyl coenzyme A.
- C. T. Evans et al. (1996), Biochemistry, 35, 10661-10672. Active site mutants of pig citrate synthase: effects of mutations on the enzyme catalytic and structural properties.
- W. Yang et al. (2003), J. Phys. Chem. B, 107, 5986-5994. Computational Study of the Citrate Synthase Catalyzed Deprotonation of Acetyl-Coenzyme A and Fluoroacetyl-Coenzyme A: Demonstration of a Layered Quantum Mechanical Approach.
Homologue information for M0078 (1al6)
MACiE Homologues (within the PDB)
MACiE Homologues (within UniprotKB/SwissProt)
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