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Overview for MACiE Entry M0077

Version history

General Information

EC Number: 2.3.1.16 (A member of the Transferases, Acyltransferases, Transferring groups other than aminoacyl groups)

Enzyme Name: acetyl-CoA C-acyltransferase

Biological Species: Saccharomyces cerevisiae (Baker's yeast)

Catalytic Chain UniprotKB Accession Codes:

  • P27796 - 3-ketoacyl-CoA thiolase, peroxisomal

Representative PDB Code: 1afw - THE 1.8 ANGSTROM CRYSTAL STRUCTURE OF THE DIMERICPEROXISOMAL THIOLASE OF SACCHAROMYCES CEREVISIAE (Resolution = 1.80 Å).

Catalytic CATH Codes:

  • 3.40.47.10 - Peroxisomal Thiolase; Chain A, domain 1

Display structure information

Overall Reaction:

Image of acetyl-CoA

Image of acyl-CoA

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Image of CoA

Image of 3-oxoacyl-CoA

acetyl-CoA
C00024
CHEBI:57288
acyl-CoA
C00040
CHEBI:37554
CoA
C00010
CHEBI:57287
3-oxoacyl-CoA
C00264
CHEBI:57347

View similar reactions


Stepwise Description of the Reaction

Step 1His375 deprotonates Cys125.
Step 2Cys125 initiates a nucleophilic attack on the carbonyl carbon of the acyl-CoA in an addition reaction.
Step 3The oxyanion collapses, eliminating CoA with concomitant deprotonation of Cys403.
Step 4Cys403 deprotonates the CH3 of the acetyl-CoA, with concomitant double bond rearrangement.
Step 5The acetyl-CoA oxyanion collapses, initiating a nucleophilic attack on the acylated Cys125 in an addition reaction.
Step 6The oxyanion collapses eliminating Cys125 with concomitant deprotonation of His375.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Cys 125 A Side Chain
His 375 A Side Chain
Cys 403 A Side Chain
Gly 405 A Main Chain Amide

References

  1. M. Mathieu et al. (1997), J. Mol. Biol., 73, 714-728. The 1.8 A crystal structure of the dimeric peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: implications for substrate binding and reaction mechanism.
    Medline: 9402066
  2. Y. Modis et al. (2000), J. Mol. Biol., 297, 1171-1182. Crystallographic analysis of the reaction pathway of Zoogloea ramigera biosynthetic thiolase.
    Medline: 10764581

Homologue information for M0077 (1afw)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0292 beta-ketoacyl-[acyl carrier protein] synthase I
2.3.1.41
1dd8 3.40.47.10
0.50.3403Compare

Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

GOA logo
catalytic activity (molecular function)
metabolic process (biological process)
transferase activity, transferring acyl groups other than amino-acyl groups (molecular function)
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