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Overview for MACiE Entry M0072

Version history

General Information

EC Number: 4.1.2.17 (A member of the Lyases, Carbon-carbon lyases, Aldehyde-lyases)

Enzyme Name: L-fuculose-phosphate aldolase

Biological Species: Escherichia coli (Bacteria)

Catalytic Chain UniprotKB Accession Codes:

  • P0AB87 - L-fuculose phosphate aldolase

Representative PDB Code: 1fua - L-FUCULOSE 1-PHOSPHATE ALDOLASE CRYSTAL FORM T (Resolution = 1.92 Å).

Catalytic CATH Codes:

Display structure information

Overall Reaction:

Image of L-fuculose 1-phosphate

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Image of L-lactaldehyde

Image of dihydroxyacetone phosphate

L-fuculose 1-phosphate
C01099
CHEBI:57268
L-lactaldehyde
C00424
CHEBI:18041
dihydroxyacetone phosphate
C00111
CHEBI:58933

Overall Comment: Dreyer et al. claim that Tyr113' is the main catalytic residue involved. However pKa studies and a Y113F mutant suggest that Glu73 is more likely [1].


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Stepwise Description of the Reaction

Step 1Ring opening of the sugar occurs outside of the enzyme active site.
Step 2Glu73 deprotonates the substrate alcohol at the C4 position, resulting in the elimination of the ene-diolate intermediate and the formation of the product lactaldehyde.
Step 3The ene-diolate intermediate deprotonates Glu73.

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Catalytic Residues Involved

Type Number Chain Location of Function
Glu 73 A Side Chain

Metal Cofactors for M0072

Type Het group Number Chain
zinc ZN 216 x Overview

References

  1. A. C. Joerger et al. (2000), Biochemistry, 39, 6033-6041. Catalytic action of fuculose 1-phosphate aldolase (class II) as derived from structure-directed mutagenesis.
    Medline: 10821675
  2. M. K. Dreyer et al. (1996), J. Mol. Biol., 259, 458-466. Catalytic mechanism of the metal-dependent fuculose aldolase from Escherichia coli as derived from the structure.
    Medline: 8676381

Homologue information for M0072 (1fua)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0273 L-ribulose-5-phosphate 4-epimerase
5.1.3.4
1jdi 3.40.225.10
0.51850.02Compare

Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

GOA logo
fucose metabolic process (biological process)
zinc ion binding (molecular function)
L-fuculose-phosphate aldolase activity (molecular function)
metal ion binding (molecular function)
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