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Entry M0065    6.3.4.4    adenylosuccinate synthase

Previous Step

Step 04

Inferred return step in which His41 deprotonates a water molecule, which in turn deprotonates Asp13.

This reaction step has been inferred

GIF of Reaction Step M0065.stg04


Comment: Distance between His41 ND1 and ASP13 OD1 is 6.72 Angstroms.



Mechanisms

Proton Transfer

Mechanism Components

Bond Cleavage
Bond Formation
Proton Relay
Enzyme Regenerated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp13 Side Chain reactant Proton Donor
Hydrogen Bond Donor
His41 Side Chain reactant Proton Acceptor
Hydrogen Bond Acceptor
Gln224 Side Chain spectator Not Active
Lys16 Side Chain spectator Not Active

Metal Cofactors involved in Step 04

Metal Type Metal Identity Chain Activity Function
magnesium MG 435 A spectator Increase Acidity
Activator

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
O-H
O-H
O-H
None
H
N
O

View similar reactions in MACiE.


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