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Entry M0065    6.3.4.4    adenylosuccinate synthase

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Step 02

Activated IMP acts as a nucleophile and attacks the gamma-phosphate of GTP in a substitution reaction. GDP and phosphorylated IMP are produced. His41 and Mg stabilise the GTP, Asp13 and Gln223 stabilise the activated IMP.

GIF of Reaction Step M0065.stg02



Mechanisms

Bimolecular Nucleophilic Substitution

Mechanism Components

Overall Reactant Used
Bond Cleavage
Bond Formation
Overall Product Formed
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp13 Side Chain spectator Hydrogen Bond Donor
Hydrogen Bond Acceptor
Electrostatic Stabiliser
His41 Side Chain spectator Electrostatic Stabiliser
Hydrogen Bond Donor
Gln224 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Lys16 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 02

Metal Type Metal Identity Chain Activity Function
magnesium MG 435 A spectator Substrate Binding
Electrostatic Stabiliser
Activator
Increase Electrophilicity

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
P-O
P-O
None
O
P

View similar reactions in MACiE.


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