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Entry M0065    6.3.4.4    adenylosuccinate synthase

Next Step

Step 01

Asp13 deprotonates IMP with concomitant tautomerisation to give the oxyanion, which is stabilised by a hydrogen bond from Gln224. Activated IMP is produced. His41 and Mg stabilise the GTP.

GIF of Reaction Step M0065.stg01



Mechanisms

Proton Transfer
Assisted Other Tautomerisation

Mechanism Components

Overall Reactant Used
Bond Order Change
Bond Cleavage
Bond Formation
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp13 Side Chain reactant Proton Acceptor
Hydrogen Bond Acceptor
His41 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Gln224 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Lys16 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
magnesium MG 435 A spectator Substrate Binding
Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
N-H
The C-N bond changes from a single to double bond
The C-O bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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