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Entry M0060    3.5.99.6    glucosamine-6-phosphate deaminase

Next Step

Step 01

In a ring opening reaction, His143 deprotonates the C1 hydroxide, cleaving the C-O bond with concomitant deprotonation of His143 by the newly formed oxyanion.

GIF of Reaction Step M0060.stg01



Mechanisms

Bimolecular Elimination
Proton Transfer

Mechanism Components

Overall Reactant Used
Bond Cleavage
Bond Formation
Bond Order Change
Intermediate Formation
Proton Relay
Decyclisation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His143 Side Chain reactant Proton Acceptor
Proton Donor
Hydrogen Bond Acceptor
Hydrogen Bond Donor
Proton Relay
Asp72 Side Chain spectator Electrostatic Stabiliser
Hydrogen Bond Acceptor
Glu148 Side Chain spectator Hydrogen Bond Acceptor
Activator
Asp141 Side Chain spectator Hydrogen Bond Acceptor
Activator

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
O-H
C-O
N-H
O-H
The C-O bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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