Overview for MACiE Entry M0058
EC Number: 184.108.40.206 (A member of the Lyases, Phosphorus-oxygen lyases, Phosphorus-oxygen lyases (only sub-subclass identified to date))
Enzyme Name: adenylate cyclase
Biological Species: Rattus norvegicus (Rat)
Catalytic Chain UniprotKB Accession Codes:
- P26769 - Adenylate cyclase type 2
Representative PDB Code: 1cju - COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIANADENYLYL CYCLASE: COMPLEX WITH BETA-L-2',3'-DIDEOXYATP ANDMG (Resolution = 2.80 Å).
Display structure information
Overall Comment: Forskolin appears to activate adenyl cyclase by promoting the assembly of the active dimer and by direct interaction within the catalytic cleft.
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Stepwise Description of the Reaction
|Step 1||The alpha-phosphate deprotonates the 3'-hydroxyl of ATP, which in turn attacks the phosphorus atom in a nucleophilic addition, forming a cyclic pentacoordinate phosphorus intermediate.|
|Step 2||The cyclic pentacoordinate phosphorus intermediate collapses eliminating the diphosphate molecule.|
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Catalytic Residues Involved
||Location of Function
Metal Cofactors for M0058
- J. J. G. Tesmer et al. (1997), Science, 278, 1907-1916. Crystal structure of the catalytic domains of adenylyl cyclase in a complex with Gsalpha.GTPgammaS.
- J. J. G. Tesmer et al. (1999), Science, 285, 756-760. Two-metal-Ion catalysis in adenylyl cyclase.
Homologue information for M0058 (1cju)
MACiE Homologues (within the PDB)
MACiE Homologues (within UniprotKB/SwissProt)
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