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Entry M0056    5.4.99.12    tRNA-pseudouridine synthase I

Next Step

Step 01

Asp39 initiates a nucleophilic attack upon the C1 of the uridine tRNA in a substitution reaction, displacing the uridine base as an anion.

GIF of Reaction Step M0056.stg01



Mechanisms

Bimolecular Nucleophilic Substitution

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Cleavage
Enzyme-Substrate Bond Formation
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Tyr67 Side Chain spectator Not Active
Asp39 Side Chain reactant Nucleophile

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
C-N
None
C
N
O

View similar reactions in MACiE.


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