Entry M0053 184.108.40.206 malate synthase
Asp631 deprotonates the methyl group of acetyl-CoA, which causes a rearrangement of the double bonds and the formation of an oxyanion.
Comment: Enzyme shows little discrimination with regard to the pro-R versus the pro-S hydrogens in fluoroacetyl-CoA, resulting in either a Z- or E-enolate intermediate. The developing negative charge is therefore stabilised by the adjacent positive charge (which is stabilised itself by the surrounding negatively charged groups in the active site) of the Arg338 .
Amino acids involved in the reaction step.
Metal Cofactors involved in Step 01
||Bonds Changed in Order
||Atom Types Involved
The C-C bond changes from a single to double bond
The C-O bond changes from a double to single bond
View similar reactions in MACiE.