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Overview for MACiE Entry M0053

Version history

General Information

EC Number: 2.3.3.9 (A member of the Transferases, Acyltransferases, Acyl groups converted into alkyl groups on transfer)

Enzyme Name: malate synthase

Biological Species: Escherichia coli (Bacteria)

Catalytic Chain UniprotKB Accession Codes:

Representative PDB Code: 1d8c - MALATE SYNTHASE G COMPLEXED WITH MAGNESIUM AND GLYOXYLATE (Resolution = 2.00 Å).

Catalytic CATH Codes:

"Other" CATH Codes:

Display structure information

Overall Reaction:

Image of oxo-acetic acid

Image of acetyl-CoA

Image of water

right arrow

Image of CoA

Image of 2-oxo-succinic acid

oxo-acetic acid
C00048
CHEBI:16891
acetyl-CoA
C00024
CHEBI:57288
water
C00001
CHEBI:15377
CoA
C00010
CHEBI:57287
2-oxo-succinic acid
C00149
CHEBI:17084

View similar reactions


Stepwise Description of the Reaction

Step 1Asp631 deprotonates the methyl group of acetyl-CoA, which causes a rearrangement of the double bonds and the formation of an oxyanion.
Step 2The CoA intermediate rotates in the active site. The oxyanion collapses, initiating a nucleophilic attack on the carbonyl carbon of the oxo-acetic acid substrate in an addition reaction.
Step 3The oxyanion deprotonates water, which initiates a nucleophilic attack on the carbonyl carbon adjacent to the sulfur of CoA in a substitution reaction, eliminating CoA with concomitant deprotonation of Asp631.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Asp 270 A Side Chain
Glu 272 A Side Chain
Arg 338 A Side Chain
Asp 631 A Side Chain

Metal Cofactors for M0053

Type Het group Number Chain
magnesium MG 3001 A Overview

References

  1. B. R. Howard et al. (2000), Biochemistry, 39, 3156-3168. Crystal structure of Escherichia coli malate synthase G complexed with magnesium and glyoxylate at 2.0 A resolution: mechanistic implications.
    Medline: 10715138
  2. C. V. Smith et al. (2003), J. Biol. Chem., 278, 1735-1743. Biochemical and structural studies of malate synthase from Mycobacterium tuberculosis.
    Medline: 12393860

Homologue information for M0053 (1d8c)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
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GOA logo
catalytic activity (molecular function)
malate synthase activity (molecular function)
glyoxylate cycle (biological process)
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