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Overview for MACiE Entry M0052

Version history

General Information

EC Number: 4.1.2.13 (A member of the Lyases, Carbon-carbon lyases, Aldehyde-lyases)

Enzyme Name: fructose-bisphosphate aldolase (Class II)

Biological Species: Escherichia coli (Bacteria)

Catalytic Chain UniprotKB Accession Codes:

  • P0AB71 - Fructose-bisphosphate aldolase class 2

Representative PDB Code: 1b57 - CLASS II FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE IN COMPLEX WITHPHOSPHOGLYCOLOHYDROXAMATE (Resolution = 2.00 Å).

Catalytic CATH Codes:

Display structure information

Overall Reaction:

Image of D-glyceraldehyde 3-phosphate

Image of glycerone phosphate

right arrow

Image of D-fructosefuranose 1,6-bisphosphate

D-glyceraldehyde 3-phosphate
C00118
CHEBI:59776
glycerone phosphate
C00111
CHEBI:57642
D-fructosefuranose 1,6-bisphosphate
C00354
CHEBI:49299

It is unknown whether this reaction is reversible or not.


View similar reactions


Stepwise Description of the Reaction

Step 1Glu182 deprotonates the C1 carbon of the substrate, which initiates double bond rearrangement to form the enol-intermediate.
Step 2The oxyanion collapses, initiating nucleophilic addition of the intermediate to the glyceraldehyde-3-phosphate at the carbonyl carbon. The oxyanion formed deprotonates Asp109.
Step 3Asp109 deprotonates water which deprotonates Glu182 in an inferred step.
Step 4The sugar cyclises outside the enzyme active site.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Asp 109 A Side Chain
Glu 182 A Side Chain
Asn 286 A Side Chain

Metal Cofactors for M0052

Type Het group Number Chain
zinc ZN 360 A Overview
sodium NA 364 A Overview

References

  1. D. R. Hall et al. (1999), J. Mol. Biol., 287, 383-394. The crystal structure of Escherichia coli class II fructose-1, 6-bisphosphate aldolase in complex with phosphoglycolohydroxamate reveals details of mechanism and specificity.
    Medline: 10080900
  2. A. R. Plater et al. (1999), J. Mol. Biol., 285, 843-855. Conserved residues in the mechanism of the E. coli Class II FBP-aldolase.
    Medline: 9878448

Homologue information for M0052 (1b57)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with the same EC number (different mechanisms):

MACiE Entry Enzyme Name PDB code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0222 fructose-bisphosphate aldolase (Class I) 2qut 0.3172 0.2454 Compare

Compare of the other reactions in MACiE with EC number 4.1.2.13


Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0102 L-lactate dehydrogenase (cytochrome)
1.1.2.3
1fcb 3.20.20.70
0.14510.18Compare
M0108 2,4-dienoyl-CoA reductase (NADPH)
1.3.1.34
1ps9 3.20.20.70
0.27110.1383Compare
M0109 dihydroorotate oxidase
1.3.3.1
1d3g 3.20.20.70
0.44440Compare
M0111 glutamate synthase (ferredoxin)
1.4.7.1
1ofd 3.20.20.70
0.35760.1579Compare
M0114 trimethylamine dehydrogenase
1.5.8.2
2tmd 3.20.20.70
0.280Compare
M0319 NADPH dehydrogenase
1.6.99.1
1oya 3.20.20.70
0.33330.2399Compare
M0148 transaldolase
2.2.1.2
1onr 3.20.20.70
0.37390.3729Compare
M0008 nicotinate-nucleotide diphosphorylase (carboxylating)
2.4.2.19
1qpr 3.20.20.70
0.50.37Compare
M0243 pyridoxine 5'-phosphate synthase
2.6.99.2
1ho1 3.20.20.70
0.34670.1563Compare
M0050 orotidine-5'-phosphate decarboxylase
4.1.1.23
1dbt 3.20.20.70
0.12820.3126Compare
M0252 indole-3-glycerol-phosphate synthase
4.1.1.48
1igs 3.20.20.70
0.51850.2822Compare
M0236 3-dehydro-L-gulonate-6-phosphate decarboxylase
4.1.1.85
1q6l 3.20.20.70
0.44440.3752Compare
M0222 fructose-bisphosphate aldolase (Class I)
4.1.2.13
2qut 3.20.20.70
0.31720.2454Compare
M0054 3-dehydroquinate dehydratase (type I)
4.2.1.10
1qfe 3.20.20.70
0.17030.195Compare
M0230 porphobilinogen synthase
4.2.1.24
1gzg 3.20.20.70
0.3160.0148Compare
M0267 dihydrodipicolinate synthase
4.2.1.52
1dhp 3.20.20.70
0.30760Compare
M0270 ribulose-phosphate 3-epimerase
5.1.3.1
1h1z 3.20.20.70
0.6250.3220Compare
M0324 triosephosphate isomerase
5.3.1.1
1tph 3.20.20.70
0.550.1499Compare
M0223 pyruvate carboxylase
6.4.1.1
2qf7 3.20.20.70
0.43580.3136Compare

View a comparison of the other reactions in MACiE with the CATH domain 3.20.20.70


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

GOA logo
catalytic activity (molecular function)
fructose-bisphosphate aldolase activity (molecular function)
glycolytic process (biological process)
metabolic process (biological process)
zinc ion binding (molecular function)
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