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Entry M0050    4.1.1.23    orotidine-5'-phosphate decarboxylase

Next Step

Step 01

The substrate undergoes decarboxylation. The substrate concomitantly deprotonates Lys62 forming UMP.

Rate Determining Step

GIF of Reaction Step M0050.stg01


Comment: The references for this reaction state that the mechanism is a SE2 however, we describe it as an E1cb and a proton transfer due to the nature of the substituting group, a proton.



Mechanisms

Unimolecular Elimination by the Conjugate Base
Proton Transfer

Mechanism Components

Overall Reactant Used
Bond Cleavage
Bond Formation
Bond Order Change
Overall Product Formed
Decarboxylation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Lys62 Side Chain reactant Proton Donor
Hydrogen Bond Donor
Asp60 Side Chain spectator Hydrogen Bond Acceptor
Repulsive Charge Charge Interaction
Electrostatic Destabiliser
Asp65B Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-H
C-C
N-H
The C-O bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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