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Entry M0049    4.1.1.22    histidine decarboxylase

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Step 06

Tyr62 deprotonates the hydroxyl group of the pyruvoyl residue, which eliminated the histamine substrate with concomitant deprotonation of the C-terminal residue.

GIF of Reaction Step M0049.stg06



Mechanisms

Bimolecular Elimination
Proton Transfer

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Intermediate Collapse
Intermediate Terminated
Enzyme-Substrate Bond Cleavage
Overall Product Formed

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Tyr62 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Glu66 Side Chain spectator Not Active
Ser81E Main Chain C Terminus reactant Proton Donor
Hydrogen Bond Donor
Hydrogen Bond Acceptor
Glu197F Side Chain spectator Hydrogen Bond Acceptor
Phe195F Main Chain Amide spectator Hydrogen Bond Donor
Pvl82F Post-Translationally Modified Residue reactant Electrofuge
Proton Donor
Hydrogen Bond Acceptor
Hydrogen Bond Donor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
O-H
C-N
O-H
O-H
The C-O bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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