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Entry M0049    4.1.1.22    histidine decarboxylase

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Step 05

The C-terminal serine residue deprotonates water, which in turn attacks the imine carbon in a nucleophilic addition, the nitrogen deprotonates Tyr62.

GIF of Reaction Step M0049.stg05



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Addition

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Intermediate Formation
Enzyme-Substrate Bond Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Tyr62 Side Chain reactant Proton Donor
Hydrogen Bond Donor
Glu66 Side Chain spectator Hydrogen Bond Acceptor
Activator
Ser81E Main Chain C Terminus reactant Proton Acceptor
Hydrogen Bond Acceptor
Glu197F Side Chain spectator Hydrogen Bond Acceptor
Phe195F Main Chain Amide spectator Hydrogen Bond Donor
Pvl82F Post-Translationally Modified Residue reactant Covalently Attached
Hydrogen Bond Acceptor
Electrophile

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
N-H
O-H
O-H
O-H
The C-N bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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