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Entry M0045    2.4.1.19    cyclomaltodextrin glucanotransferase

Previous Step

Step 02

Glu257 deprotonates a the free sugar molecule, which initiates a nucleophilic attack upon the covalently bound sugar in a substitution reaction, eliminating Asp229.

GIF of Reaction Step M0045.stg02



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Substitution

Mechanism Components

Bond Cleavage
Bond Formation
Overall Product Formed
Enzyme-Substrate Bond Cleavage
Enzyme Regenerated
Intermediate Terminated
Intermediate Collapse

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp229 Side Chain reactant Nucleofuge
Arg227 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
His327 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asp328 Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Glu257 Side Chain reactant Proton Acceptor
Hydrogen Bond Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
O-H
C-O
O-H
None
C
H
O

View similar reactions in MACiE.


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