spacer

Entry M0045    2.4.1.19    cyclomaltodextrin glucanotransferase

Next Step

Step 01

Asp229 initiates a nucleophilic attack upon the carbon of the ether linkage between the two sugar rings in a substitution reaction, which eliminates a free sugar molecule, with concomitant deprotonation of Glu257.

GIF of Reaction Step M0045.stg01



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Substitution

Mechanism Components

Overall Reactant Used
Bond Cleavage
Bond Formation
Enzyme-Substrate Bond Formation
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp229 Side Chain reactant Nucleophile
Arg227 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
His327 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asp328 Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Glu257 Side Chain reactant Proton Donor
Hydrogen Bond Donor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
O-H
C-O
O-H
None
C
H
O

View similar reactions in MACiE.


spacer
spacer