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Overview for MACiE Entry M0045

Version history

General Information

EC Number: 2.4.1.19 (A member of the Transferases, Glycosyltransferases, Hexosyltransferases)

Enzyme Name: cyclomaltodextrin glucanotransferase

Biological Species: Bacillus circulans (Bacteria)

Catalytic Chain UniprotKB Accession Codes:

  • P43379 - Cyclomaltodextrin glucanotransferase

Representative PDB Code: 1cdg - NUCLEOTIDE SEQUENCE AND X-RAY STRUCTURE OF CYCLODEXTRINGLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 INA MALTOSE-DEPENDENT CRYSTAL FORM (Resolution = 2.00 Å).

Catalytic CATH Codes:

"Other" CATH Codes:

Display structure information

Overall Reaction:

Image of Sugar

Image of 1,4-alpha-D-glucan chain

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Image of 1,4-alpha-D-glucan chain

Image of Sugar

Sugar
C11477
CHEBI:16646
1,4-alpha-D-glucan chain
C00718
CHEBI:15444
1,4-alpha-D-glucan chain
C00718
CHEBI:15444
Sugar
C11477
CHEBI:16646

Overall Comment: This enzyme catalyses the hydrolysis of the alpha 1-4 glycosidic bonds in oligosaccharides and reformation to make cyclic oligosaccharides.


View similar reactions


Stepwise Description of the Reaction

Step 1Asp229 initiates a nucleophilic attack upon the carbon of the ether linkage between the two sugar rings in a substitution reaction, which eliminates a free sugar molecule, with concomitant deprotonation of Glu257.
Step 2Glu257 deprotonates a the free sugar molecule, which initiates a nucleophilic attack upon the covalently bound sugar in a substitution reaction, eliminating Asp229.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Arg 227 A Side Chain
Asp 229 A Side Chain
Glu 257 A Side Chain
His 327 A Side Chain
Asp 328 A Side Chain

References

  1. J. C. M. Uitdehaag et al. (1996), Nat. Struct. Biol., 6, 432-436. X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family.
    Medline: 10331869

Homologue information for M0045 (1cdg)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0285 oligo-1,6-glucosidase
3.2.1.10
1uok 3.20.20.80
2.60.40.1180
10.8237Compare

Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

GOA logo
catalytic activity (molecular function)
extracellular region (cellular component)
carbohydrate metabolic process (biological process)
transferase activity (molecular function)
transferase activity, transferring glycosyl groups (molecular function)
carbohydrate binding (molecular function)
cation binding (molecular function)
cyclomaltodextrin glucanotransferase activity (molecular function)
metal ion binding (molecular function)
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