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Overview for MACiE Entry M0044

Version history

General Information

EC Number: 3.1.3.1 (A member of the Hydrolases, Acting on ester bonds, Phosphoric-monoester hydrolases)

Enzyme Name: alkaline phosphatase

Biological Species: Escherichia coli (Bacteria)

Catalytic Chain UniprotKB Accession Codes:

Representative PDB Code: 1alk - REACTION MECHANISM OF ALKALINE PHOSPHATASE BASED ON CRYSTALSTRUCTURES. TWO METAL ION CATALYSIS (Resolution = 2.00 Å).

Catalytic CATH Codes:

Display structure information

Overall Reaction:

Image of phosphoric monoester

Image of water

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Image of alcohol

Image of phosphate

phosphoric monoester
C01153
water
C00001
CHEBI:15377
alcohol
C00069
CHEBI:30879
phosphate
C00009
CHEBI:18367

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Stepwise Description of the Reaction

Step 1Metal activated water deprotonates Ser102.
Step 2Ser102 initiates a nucleophilic attack on the phosphoric monoester in a substitution reaction which eliminates the alcohol as an anionic species.
Step 3The alcohol anion deprotonates water, which initiates a nucleophilic attack on the covalently bound phosphorous in a substitution reaction which eliminates serine as an anion.
Step 4Ser102 deprotonates water to regenerate the starting state of the enzyme.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Ser 102 A Side Chain
Arg 166 A Side Chain

Metal Cofactors for M0044

Type Het group Number Chain
zinc ZN 450 A Overview
zinc ZN 451 A Overview
magnesium MG 452 A Overview

References

  1. B. Stec et al. (2000), J. Mol. Biol., 299, 1303-1311. A revised mechanism for the alkaline phosphatase reaction involving three metal ions.
    Medline: 10873454
  2. L. Ma et al. (1994), J. Biol. Chem., 269, 31614-31619. Mutations at histidine 412 alter zinc binding and eliminate transferase activity in Escherichia coli alkaline phosphatase.
    Medline: 7989332
  3. J. E. Murphy et al. (1997), Nat. Struct. Biol., 4, 618-622. Trapping and visualization of a covalent enzyme-phosphate intermediate.
    Medline: 9253408

Homologue information for M0044 (1alk)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0158 cerebroside-sulfatase
3.1.6.8
1auk 3.40.720.10
0.45450.1773Compare

Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ

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catalytic activity (molecular function)
metabolic process (biological process)
dephosphorylation (biological process)
phosphatase activity (molecular function)
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