Overview for MACiE Entry M0044
EC Number: 220.127.116.11 (A member of the Hydrolases, Acting on ester bonds, Phosphoric-monoester hydrolases)
Enzyme Name: alkaline phosphatase
Biological Species: Escherichia coli (Bacteria)
Catalytic Chain UniprotKB Accession Codes:
Representative PDB Code: 1alk - REACTION MECHANISM OF ALKALINE PHOSPHATASE BASED ON CRYSTALSTRUCTURES. TWO METAL ION CATALYSIS (Resolution = 2.00 Å).
Catalytic CATH Codes:
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Stepwise Description of the Reaction
|Step 1||Metal activated water deprotonates Ser102.|
|Step 2||Ser102 initiates a nucleophilic attack on the phosphoric monoester in a substitution reaction which eliminates the alcohol as an anionic species.|
|Step 3||The alcohol anion deprotonates water, which initiates a nucleophilic attack on the covalently bound phosphorous in a substitution reaction which eliminates serine as an anion.|
|Step 4||Ser102 deprotonates water to regenerate the starting state of the enzyme.|
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Catalytic Residues Involved
||Location of Function
Metal Cofactors for M0044
- B. Stec et al. (2000), J. Mol. Biol., 299, 1303-1311. A revised mechanism for the alkaline phosphatase reaction involving three metal ions.
- L. Ma et al. (1994), J. Biol. Chem., 269, 31614-31619. Mutations at histidine 412 alter zinc binding and eliminate transferase activity in Escherichia coli alkaline phosphatase.
- J. E. Murphy et al. (1997), Nat. Struct. Biol., 4, 618-622. Trapping and visualization of a covalent enzyme-phosphate intermediate.
Homologue information for M0044 (1alk)
MACiE Homologues (within the PDB)
MACiE Homologues (within UniprotKB/SwissProt)
Entries with at least one Catalytic CATH code in common (different mechanisms):
|M0158 ||cerebroside-sulfatase |
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