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Entry M0041    3.1.21.1    deoxyribonuclease I

Previous Step

Step 02

His134 deprotonates water, which in turn deprotonates His252 in an inferred return step.

This reaction step has been inferred

GIF of Reaction Step M0041.stg02


Comment: The N on His252 is 5.7 Angstroms away from the N on His 134.



Mechanisms

Proton Transfer

Mechanism Components

Bond Cleavage
Bond Formation
Enzyme Regenerated
Proton Relay

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His134 Side Chain reactant Proton Acceptor
Hydrogen Bond Donor
Hydrogen Bond Acceptor
Glu78 Side Chain spectator Hydrogen Bond Acceptor
Activator
His252 Side Chain reactant Proton Donor
Hydrogen Bond Donor
Hydrogen Bond Acceptor
Tyr76 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asp212 Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser

Metal Cofactors involved in Step 02

Metal Type Metal Identity Chain Activity Function
magnesium MG(not in PDB) 1 x spectator Not Active
magnesium MG(not in PDB) 2 x spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
O-H
N-H
O-H
None
H
N
O

View similar reactions in MACiE.


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