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Entry M0040    2.7.2.3    phosphoglycerate kinase

Step 01

The beta-phosphate of ADP initiates a nucleophilic attack on the 1-phosphate of 1,3-biphosphoglycerate in a substitution reaction, eliminating 3-phosphoglycate.

Rate Determining Step

GIF of Reaction Step M0040.stg01


Comment: Lys219 guides the phosphate group to its substrate.



Mechanisms

Bimolecular Nucleophilic Substitution

Mechanism Components

Overall Reactant Used
Bond Cleavage
Bond Formation
Overall Product Formed

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg39 Side Chain spectator Electrostatic Stabiliser
Hydrogen Bond Donor
Lys219 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Gly376 Main Chain Amide spectator Electrostatic Stabiliser
Hydrogen Bond Donor
Gly399 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
magnesium MG 422 A spectator Substrate Binding
Electrostatic Stabiliser
Activator
Increase Electrophilicity

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
P-O
P-O
None
O
P

View similar reactions in MACiE.


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