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Entry M0036    3.8.1.2    (S)-2-haloacid dehalogenase

Next Step

Step 01

Asp8 initiates a nucleophilic attack upon the chlorinated carbon of the substrate in a substitution reaction, eliminating chloride.

GIF of Reaction Step M0036.stg01



Mechanisms

Bimolecular Nucleophilic Substitution

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Cleavage
Overall Product Formed
Enzyme-Substrate Bond Formation
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp176 Side Chain spectator Hydrogen Bond Acceptor
Activator
Arg39 Side Chain spectator Hydrogen Bond Donor
Lys147 Side Chain spectator Hydrogen Bond Donor
Activator
Ser171 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Thr12 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asn173 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Asp8 Side Chain reactant Nucleophile
Hydrogen Bond Acceptor
Phe175 Side Chain spectator Polar Non-polar interaction
Asn115 Side Chain spectator Hydrogen Bond Donor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
C-Cl
None
C
Cl
O

View similar reactions in MACiE.


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