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Overview for MACiE Entry M0035

Version history

General Information

EC Number: 2.7.11.19 (A member of the Transferases, Transferring phosphorus-containing groups, Protein-serine/threonine kinases)

Enzyme Name: phosphorylase kinase

Biological Species: Oryctolagus cuniculus (Rabbit)

Catalytic Chain UniprotKB Accession Codes:

  • P00518 - Phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform

Representative PDB Code: 2phk - THE CRYSTAL STRUCTURE OF A PHOSPHORYLASE KINASE PEPTIDE SUBSTRATECOMPLEX: KINASE SUBSTRATE RECOGNITION (Resolution = 2.60 Å).

Catalytic CATH Codes:

"Other" CATH Codes:

Display structure information

Overall Reaction:

Image of phosphorylase b

Image of ATP

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Image of ADP

Image of phosphorylase a

phosphorylase b
C02308
ATP
C00002
CHEBI:30616
ADP
C00008
CHEBI:456216
phosphorylase a
C02307

Overall Comment: The enzyme has a high affinity and low affinity binding site, both Mg(II) and Mn(II) activate the enzyme at the high affinity site, however, only Mg(II) activates the enzyme at the low affinity site.


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Stepwise Description of the Reaction

Step 1Asp149 deprotonates the protein substrate hydroxyl group, which initiates a nucleophilic attack upon the gamma-phosphate of ATP in a substitution reaction that eliminates ADP.
Step 2The phospohrylated protein deprotonates Asp149.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Asp 149 A Side Chain
Lys 151 A Side Chain
Thr 186 A Side Chain

Metal Cofactors for M0035

Type Het group Number Chain
magnesium MN 382 x Overview
none MN 383 x Overview

References

  1. V. T. Skamnaki et al. (1999), Biochemistry, 38, 14718-14730. Catalytic mechanism of phosphorylase kinase probed by mutational studies.
    Medline: 10545198
  2. E. D. Lowe et al. (1997), The EMBO Journal, 16, 6646-6658. The crystal structure of a phosphorylase kinase peptide substrate complex: kinase substrate recognition.
    Medline: 9362479
  3. D. J. Owen et al. (1995), Structure, 3, 467-482. Two structures of the catalytic domain of phosphorylase kinase: an active protein kinase complexed with substrate analogue and product.
    Medline: 7663944

Homologue information for M0035 (2phk)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)



Entries with at least one Catalytic CATH code in common (different mechanisms):

MACiE Entry Enzyme Name
EC Number
PDB code CATH code Composite
Reaction Similarity
Catalytic Machinery
Similarity
M0246 receptor protein-tyrosine kinase
2.7.10.1
1ir3 1.10.510.10
3.30.200.20
0.53840.253Compare
M0282 mitogen-activated protein kinase kinase
2.7.12.2
1s9i 1.10.510.10
3.30.200.20
0.70.3792Compare

View a comparison of the other reactions in MACiE with the CATH domain 1.10.510.10

View a comparison of the other reactions in MACiE with the CATH domain 3.30.200.20


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

GOA logo
nucleotide binding (molecular function)
protein kinase activity (molecular function)
protein serine/threonine kinase activity (molecular function)
phosphorylase kinase activity (molecular function)
calmodulin binding (molecular function)
ATP binding (molecular function)
phosphorylase kinase complex (cellular component)
carbohydrate metabolic process (biological process)
glycogen metabolic process (biological process)
glycogen biosynthetic process (biological process)
protein phosphorylation (biological process)
kinase activity (molecular function)
phosphorylation (biological process)
transferase activity (molecular function)
transferase activity, transferring phosphorus-containing groups (molecular function)
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