Overview for MACiE Entry M0035
EC Number: 22.214.171.124 (A member of the Transferases, Transferring phosphorus-containing groups, Protein-serine/threonine kinases)
Enzyme Name: phosphorylase kinase
Biological Species: Oryctolagus cuniculus (Rabbit)
Catalytic Chain UniprotKB Accession Codes:
- P00518 - Phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform
Representative PDB Code: 2phk - THE CRYSTAL STRUCTURE OF A PHOSPHORYLASE KINASE PEPTIDE SUBSTRATECOMPLEX: KINASE SUBSTRATE RECOGNITION (Resolution = 2.60 Å).
Catalytic CATH Codes:
"Other" CATH Codes:
Display structure information
Overall Comment: The enzyme has a high affinity and low affinity binding site, both Mg(II) and Mn(II) activate the enzyme at the high affinity site, however, only Mg(II) activates the enzyme at the low affinity site.
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Stepwise Description of the Reaction
|Step 1||Asp149 deprotonates the protein substrate hydroxyl group, which initiates a nucleophilic attack upon the gamma-phosphate of ATP in a substitution reaction that eliminates ADP.|
|Step 2||The phospohrylated protein deprotonates Asp149.|
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Catalytic Residues Involved
||Location of Function
Metal Cofactors for M0035
- V. T. Skamnaki et al. (1999), Biochemistry, 38, 14718-14730. Catalytic mechanism of phosphorylase kinase probed by mutational studies.
- E. D. Lowe et al. (1997), The EMBO Journal, 16, 6646-6658. The crystal structure of a phosphorylase kinase peptide substrate complex: kinase substrate recognition.
- D. J. Owen et al. (1995), Structure, 3, 467-482. Two structures of the catalytic domain of phosphorylase kinase: an active protein kinase complexed with substrate analogue and product.
Homologue information for M0035 (2phk)
MACiE Homologues (within the PDB)
MACiE Homologues (within UniprotKB/SwissProt)
Entries with at least one Catalytic CATH code in common (different mechanisms):
|M0246 ||receptor protein-tyrosine kinase |
|M0282 ||mitogen-activated protein kinase kinase |
View a comparison of the other reactions in MACiE with the CATH domain 1.10.510.10
View a comparison of the other reactions in MACiE with the CATH domain 126.96.36.199
Links to this entry in other databases