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Entry M0030    2.3.1.54    formate C-acetyltransferase

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Step 06

CoA initiates a homolytic attack upon the carbonyl carbon of the acyl-Cys418 in a substitution reaction, eliminating Cys418, which abstracts a hydrogen from Cys419.

GIF of Reaction Step M0030.stg06



Mechanisms

Radical Propagation
Hydrogen Transfer
Bimolecular Homolytic Substitution

Mechanism Components

Bond Cleavage
Bond Formation
Overall Product Formed
Enzyme-Substrate Bond Cleavage
Intermediate Terminated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Cys419 Side Chain reactant Hydrogen Donor
Hydrogen Bond Acceptor
Hydrogen Bond Donor
Trp333 Side Chain spectator Hydrogen Bond Donor
Cys418 Side Chain reactant Leaving Group
Hydrogen Acceptor
Hydrogen Bond Acceptor
Gly734 Main Chain Alpha Carbon spectator Hydrogen Bond Donor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-S
S-H
C-S
S-H
None
C
H
S

View similar reactions in MACiE.


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