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Entry M0030    2.3.1.54    formate C-acetyltransferase

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Step 02

Cys418 initiates a homolytic attack on the carbonyl carbon of the pyruvate substrate in an addition reaction.

GIF of Reaction Step M0030.stg02



Mechanisms

Radical Propagation
Bimolecular Homolytic Addition

Mechanism Components

Overall Reactant Used
Bond Formation
Bond Order Change
Enzyme-Substrate Bond Formation
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Cys419 Side Chain spectator Hydrogen Bond Donor
Hydrogen Bond Acceptor
Trp333 Side Chain spectator Radical Stabiliser
Hydrogen Bond Donor
Cys418 Side Chain reactant Hydrogen Bond Acceptor
Radical Combinant
Gly734 Main Chain Alpha Carbon spectator Hydrogen Bond Donor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-S
None
The C-O bond changes from a double to single bond
C
O
S

View similar reactions in MACiE.


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