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Entry M0029    3.5.1.38    glutamin-(asparagin-)ase

Previous Step

Step 04

Glu3294B deprotonates the hydroxyl formed from the oxyanion, initiating the elimination of Thr1020, which in turn deprotonates Tyr1034, which then deprotonates Glu3294B.

GIF of Reaction Step M0029.stg04



Mechanisms

Bimolecular Elimination
Proton Transfer

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Proton Relay
Overall Product Formed
Enzyme Regenerated
Enzyme-Substrate Bond Cleavage
Intermediate Collapse
Intermediate Terminated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Tyr1034 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Hydrogen Bond Donor
Proton Donor
Proton Relay
Lys1173 Side Chain spectator Hydrogen Bond Donor
Hydrogen Bond Acceptor
Glu3294B Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Proton Donor
Proton Relay
Asp1101 Side Chain spectator Hydrogen Bond Acceptor
Thr1100 Side Chain spectator Hydrogen Bond Donor
Thr1020 Side Chain reactant Nucleofuge
Hydrogen Bond Acceptor
Proton Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
O-H
O-H
C-O
O-H
O-H
O-H
The C-O bond changes from a single to double bond
C
H
O

View similar reactions in MACiE.


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