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Entry M0029    3.5.1.38    glutamin-(asparagin-)ase

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Step 02

Glu3294B deprotonates the hydroxyl formed from the oxyanion, initiating the elimination of ammonia, which gains a proton from Glu3294B.

GIF of Reaction Step M0029.stg02



Mechanisms

Proton Transfer
Bimolecular Elimination

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Overall Product Formed
Proton Relay
Deamination
Enzyme-Substrate Bond Cleavage
Intermediate Collapse
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Tyr1034 Side Chain reactant Proton Donor
Proton Acceptor
Proton Relay
Hydrogen Bond Acceptor
Hydrogen Bond Donor
Lys1173 Side Chain spectator Hydrogen Bond Donor
Hydrogen Bond Acceptor
Glu3294B Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Proton Donor
Proton Relay
Asp1101 Side Chain spectator Hydrogen Bond Acceptor
Thr1100 Side Chain spectator Hydrogen Bond Donor
Hydrogen Bond Acceptor
Thr1020 Side Chain reactant Covalently Attached
Hydrogen Bond Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
O-H
C-N
O-H
O-H
The C-O bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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