Overview for MACiE Entry M0027
EC Number: 18.104.22.168 (A member of the Hydrolases, Acting on ester bonds, Phosphoric-diester hydrolases)
Enzyme Name: phospholipase C
Biological Species: Bacillus cereus (Bacteria)
Catalytic Chain UniprotKB Accession Codes:
Representative PDB Code: 1ah7 - PHOSPHOLIPASE C FROM BACILLUS CEREUS (Resolution = 1.50 Å).
Display structure information
This reaction is irreversible.
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Stepwise Description of the Reaction
|Step 1||Asp55 deprotonates water, which initiates nucleophilic attack on the phosphate in a substitution reaction, eliminating 1,2-diacylglycerol.|
|Step 2||the phosphate of choline phosphate deprotonates Asp55 in an inferred return step.|
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Catalytic Residues Involved
||Location of Function
Metal Cofactors for M0027
- S. F. Martin et al. (1999), Biochemistry, 38, 4403-4408. Catalytic cycle of the phosphatidylcholine-preferring phospholipase C from Bacillus cereus. Solvent viscosity, deuterium isotope effects, and proton inventory studies.
Homologue information for M0027 (1ah7)
MACiE Homologues (within the PDB)
MACiE Homologues (within UniprotKB/SwissProt)
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