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Entry M0021    1.1.1.38    malate dehydrogenase (oxaloacetate-decarboxylating)

Previous Step

Step 04

Tyr112 deprotonates Lys183 which deprotonates Asp278 in an inferred return step.

This reaction step has been inferred

GIF of Reaction Step M0021.stg04


Comment: We assume a proton relay through Lys183 as Asp278 and Tyr112 are over 5.5 Angstroms apart.



Mechanisms

Proton Transfer

Mechanism Components

Proton Relay
Enzyme Regenerated
Bond Formation
Bond Cleavage

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Arg165 Side Chain spectator Not Active
Lys183 Side Chain reactant Proton Acceptor
Proton Donor
Hydrogen Bond Donor
Asp278 Side Chain reactant Proton Donor
Hydrogen Bond Acceptor
Tyr112 Side Chain reactant Proton Acceptor
Hydrogen Bond Acceptor
Asn421 Side Chain spectator Not Active

Metal Cofactors involved in Step 04

Metal Type Metal Identity Chain Activity Function
manganese MN 604 x spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
O-H
N-H
O-H
None
H
N
O

View similar reactions in MACiE.


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