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Entry M0021    1.1.1.38    malate dehydrogenase (oxaloacetate-decarboxylating)

Next Step

Step 01

Asp278 deprotonates Lys183, which deprotonates the hydroxyl group of malate, which eliminates a hydride ion that is added to NADP.

GIF of Reaction Step M0021.stg01



Mechanisms

Hydride Transfer
Bimolecular Elimination
Aromatic Bimolecular Nucleophilic Addition

Mechanism Components

Overall Reactant Used
Bond Cleavage
Bond Formation
Bond Order Change
Overall Product Formed
Intermediate Formation
Proton Relay

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp278 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Lys183 Side Chain reactant Proton Acceptor
Proton Donor
Proton Relay
Hydrogen Bond Donor
Asn421 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Arg165 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Tyr112 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 01

Metal Type Metal Identity Chain Activity Function
manganese MN 604 x spectator Substrate Binding
Increase Acidity
Electrostatic Stabiliser
Activator

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-H
N-H
O-H
C-H
N-H
O-H
The C-C bond changes from a single to double bond
The C-O bond changes from a single to double bond
The C-C bond changes from a double to single bond
The C-N bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


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