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Entry M0019    3.2.1.113    mannosyl-oligosaccharide 1,2-alpha-mannosidase

Previous Step

Step 02

In an inferred step, Asp275 and Glu132 are returned to their starting states through a proton relay chain of water molecules.

This reaction step has been inferred

GIF of Reaction Step M0019.stg02


Comment: The number of waters in proton relay unknown. Distance between Asp275 and Glu132 is approximately 10 Angstroms.



Mechanisms

Proton Transfer

Mechanism Components

Proton Relay
Enzyme Regenerated
Bond Formation
Bond Cleavage

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp275 Side Chain reactant Proton Donor
Hydrogen Bond Donor
Glu132 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Arg136 Side Chain spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Metal Cofactors involved in Step 02

Metal Type Metal Identity Chain Activity Function
calcium CA 901 x spectator Not Active

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
O-H
O-H
O-H
O-H
O-H
O-H
O-H
None
H
O

View similar reactions in MACiE.


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