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Entry M0018    2.1.4.1    glycine amidinotransferase

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Step 02

His303 deprotonates the amine of the substrate glycine, which initiates a nucleophilic attack on the covalently bound carbon in a substitution reaction, eliminating Cys407, which deprotonates the substrate glycine.

GIF of Reaction Step M0018.stg02



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Substitution

Mechanism Components

Overall Reactant Used
Overall Product Formed
Bond Cleavage
Bond Formation
Enzyme-Substrate Bond Cleavage
Intermediate Collapse
Intermediate Terminated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His303 Side Chain reactant Proton Acceptor
Hydrogen Bond Acceptor
Hydrogen Bond Donor
Asp305 Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Asp170 Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Asp254 Side Chain spectator Hydrogen Bond Acceptor
Activator
Cys407 Side Chain reactant Nucleofuge
Hydrogen Bond Acceptor
Proton Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-N
N-H
S-H
C-S
N-H
N-H
None
C
H
N
S

View similar reactions in MACiE.


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