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Entry M0018    2.1.4.1    glycine amidinotransferase

Next Step

Step 01

The substrate arginine deprotonates Cys407, which initiates a nucleophilic attack on the guanido carbon of the arginine in a substitution reaction which eliminates ornithine.

GIF of Reaction Step M0018.stg01



Mechanisms

Proton Transfer
Bimolecular Nucleophilic Substitution

Mechanism Components

Overall Reactant Used
Bond Cleavage
Bond Formation
Enzyme-Substrate Bond Formation
Overall Product Formed
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
His303 Side Chain spectator Hydrogen Bond Donor
Hydrogen Bond Acceptor
Asp305 Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Asp170 Side Chain spectator Hydrogen Bond Acceptor
Electrostatic Stabiliser
Cys407 Side Chain reactant Nucleophile
Hydrogen Bond Donor
Proton Donor
Asp254 Side Chain spectator Hydrogen Bond Acceptor

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-S
N-H
C-N
S-H
None
C
H
N
S

View similar reactions in MACiE.


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