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Overview for MACiE Entry M0018

Version history

General Information

EC Number: 2.1.4.1 (A member of the Transferases, Transferring one-carbon groups, Amidinotransferases)

Enzyme Name: glycine amidinotransferase

Biological Species: Homo sapiens (Human)

Catalytic Chain UniprotKB Accession Codes:

  • P50440 - Glycine amidinotransferase, mitochondrial

Representative PDB Code: 1jdw - CRYSTAL STRUCTURE AND MECHANISM OF L-ARGININE: GLYCINEAMIDINOTRANSFERASE: A MITOCHONDRIAL ENZYME INVOLVED INCREATINE BIOSYNTHESIS (Resolution = 1.90 Å).

Catalytic CATH Codes:

  • 3.75.10.10 - L-arginine/glycine Amidinotransferase; Chain A

Display structure information

Overall Reaction:

Image of L-arginine

Image of glycine

right arrow

Image of proton

Image of L-ornithine

Image of guanidoacetic acid

L-arginine
C00062
CHEBI:32682
glycine
C00037
CHEBI:57305
proton
C00080
CHEBI:24636
L-ornithine
C00077
CHEBI:15729
guanidoacetic acid
C00581
CHEBI:57742

View similar reactions


Stepwise Description of the Reaction

Step 1The substrate arginine deprotonates Cys407, which initiates a nucleophilic attack on the guanido carbon of the arginine in a substitution reaction which eliminates ornithine.
Step 2His303 deprotonates the amine of the substrate glycine, which initiates a nucleophilic attack on the covalently bound carbon in a substitution reaction, eliminating Cys407, which deprotonates the substrate glycine.
Step 3Water deprotonates His303 in an inferred return step.

View similar reactions (composite manual annotation)


Catalytic Residues Involved

Type Number Chain Location of Function
Asp 170 A Side Chain
Asp 254 A Side Chain
His 303 A Side Chain
Asp 305 A Side Chain
Cys 407 A Side Chain

References

  1. H. Shirai et al. (2001), Trends Biochem. Sci., 26, 465-468. A novel superfamily of enzymes that catalyze the modification of guanidino groups.
    Medline: 11504612

Homologue information for M0018 (1jdw)

CSA Homologues

MACiE Homologues (within the PDB)

MACiE Homologues (within UniprotKB/SwissProt)


Links to this entry in other databases

Link to EC-PDB-SUM Link to PDB-SUM Link to RCSB PDB Link to PDBe Link to CSA
Link to MetaCyc Link to KEGG Link to BRENDA Link to ExplorENZ
Link to EzCatDB

GOA logo
protein binding (molecular function)
cytoplasm (cellular component)
mitochondrion (cellular component)
mitochondrial inner membrane (cellular component)
mitochondrial intermembrane space (cellular component)
creatine metabolic process (biological process)
creatine biosynthetic process (biological process)
response to oxidative stress (biological process)
response to nutrient (biological process)
embryo development (biological process)
response to organic substance (biological process)
glycine amidinotransferase activity (molecular function)
membrane (cellular component)
transferase activity (molecular function)
cellular nitrogen compound metabolic process (biological process)
tissue regeneration (biological process)
response to peptide hormone (biological process)
response to mercury ion (biological process)
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