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Entry M0013    1.4.99.3    amine dehydrogenase

Previous Step

Step 12

Ammonia deprotonates Asp32 and water deprotonates Asp76 in an inferred return step.

This reaction step has been inferred

GIF of Reaction Step M0013.stg12


Comment: Inferred step added to regenerate Asp32 and Asp76 and to produce correct overall product, ammonium (C01342).



Mechanisms

Proton Transfer

Mechanism Components

Bond Formation
Bond Cleavage
Enzyme Regenerated
Overall Product Formed
Overall Reactant Used
Intermediate Terminated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp76 Side Chain reactant Hydrogen Bond Donor
Proton Donor
Asp32 Side Chain reactant Hydrogen Bond Donor
Proton Donor
Trp57 Post-Translationally Modified Residue spectator Covalently Attached
Trp108 Post-Translationally Modified Residue spectator Covalently Attached

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
O-H
O-H
O-H
None
H
N
O

View similar reactions in MACiE.


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