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Entry M0013    1.4.99.3    amine dehydrogenase

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Step 11

The amine nitrogen deprotonates the newly added hydroxyl group, which eliminates ammonia.

GIF of Reaction Step M0013.stg11


Comment: The alternative mechanism for this step may be a base activated deamination.



Mechanisms

Intramolecular Elimination

Mechanism Components

Bond Formation
Bond Cleavage
Enzyme-Substrate Bond Cleavage
Bond Order Change
Cofactor Regenerated
Intermediate Collapse
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp76 Side Chain spectator Hydrogen Bond Donor
Hydrogen Bond Acceptor
Asp32 Side Chain spectator Hydrogen Bond Donor
Trp57 Post-Translationally Modified Residue reactant Covalently Attached
Hydrogen Bond Donor
Proton Donor
Electrofuge
Trp108 Post-Translationally Modified Residue spectator Covalently Attached

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
C-N
O-H
The C-O bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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