spacer

Entry M0013    1.4.99.3    amine dehydrogenase

Previous Step     Next Step

Step 10

The amide nitrogen deprotonates water, which initiates a nucleophilic attack on the carbon of the cofactor in an addition reaction.

GIF of Reaction Step M0013.stg10


Comment: The alternative mechanism for this step may be a base activated addition of water.



Mechanisms

Bimolecular Nucleophilic Addition
Proton Transfer

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Intermediate Formation
Enzyme-Substrate Bond Formation
Overall Reactant Used

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp76 Side Chain spectator Hydrogen Bond Acceptor
Hydrogen Bond Donor
Asp32 Side Chain spectator Hydrogen Bond Donor
Trp57 Post-Translationally Modified Residue reactant Covalently Attached
Electrophile
Hydrogen Bond Acceptor
Proton Acceptor
Trp108 Post-Translationally Modified Residue spectator Covalently Attached

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
C-O
N-H
O-H
The C-N bond changes from a double to single bond
C
H
N
O

View similar reactions in MACiE.


spacer
spacer