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Entry M0013    1.4.99.3    amine dehydrogenase

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Step 07

Asp32 deprotonates the newly added hydroxyl group, eliminating the cofactor with concomitant deprotonation of Asp76, the formaldehyde products is released.

GIF of Reaction Step M0013.stg07


Comment: The identities of the bases in this step are assumed from structural alignment with QHNDH.



Mechanisms

Bimolecular Elimination
Proton Transfer

Mechanism Components

Bond Formation
Bond Cleavage
Bond Order Change
Enzyme-Substrate Bond Cleavage
Overall Product Formed
Intermediate Collapse
Intermediate Formation

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Asp32 Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Asp76 Side Chain reactant Hydrogen Bond Donor
Proton Donor
Trp57 Post-Translationally Modified Residue reactant Covalently Attached
Hydrogen Bond Donor
Proton Donor
Hydrogen Bond Acceptor
Proton Acceptor
Nucleofuge
Trp108 Post-Translationally Modified Residue spectator Covalently Attached

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
N-H
O-H
C-N
O-H
O-H
The C-O bond changes from a single to double bond
C
H
N
O

View similar reactions in MACiE.


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