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Entry M0002    3.5.2.6    beta-lactamase (Class A)

Previous Step

Step 05

The tetrahedral intermediate collapses, cleaving the acyl-enzyme bond and liberating Ser70, which in turn deprotonates the Glu166.

GIF of Reaction Step M0002.stg05



Mechanisms

Proton Transfer
Unimolecular Elimination by the Conjugate Base

Mechanism Components

Bond Cleavage
Bond Formation
Bond Order Change
Overall Product Formed
Enzyme-Substrate Bond Cleavage
Enzyme Regenerated
Intermediate Collapse
Intermediate Terminated

Amino acids involved in the reaction step.

Amino Acid Location of Function Activity Function
Lys73 Side Chain spectator Hydrogen Bond Donor
Ser130 Side Chain spectator Hydrogen Bond Donor
Hydrogen Bond Acceptor
Ser70A Side Chain reactant Hydrogen Bond Acceptor
Proton Acceptor
Nucleofuge
Glu166 Side Chain reactant Hydrogen Bond Donor
Proton Donor
Hydrogen Bond Acceptor
Ser70A Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser
Ala237 Main Chain Amide spectator Hydrogen Bond Donor
Electrostatic Stabiliser

Reactive Centre

Bonds Formed Bonds Cleaved Bonds Changed in Order Atom Types Involved
O-H
C-O
O-H
The C-O bond changes from a single to double bond
C
H
O

View similar reactions in MACiE.


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