spacer

MG 1156 B

Metal-MACiE entry for MG 1156 B

This is one of the two essential Mg ions bound to the N-terminal biotin carboxylase domain. This two ions activate ATP to the hydrolysis PMID17717183.


Metal in MACiE Physiological Metal Site Type
magnesium magnesium mononuclear

Resting State

There is no PDB code associated with this metal atom in the enzyme's resiting state.

MACiE Metal Type
(Identity)
magnesium
(MG1156B)
Oxidation State 2
Coordination Geometry
(Number of ligands)
octahedral
(6)

The resting state enzyme should not bind the Mg-ATP cofactor.

This step has no available structures in the PDB: all the coordinating information are derived from the reaction model in MACiE.


Step 01

Metal Functions

Substrate Binding

Electrostatic Stabiliser

Activator. Note: It activates ATP.

Increase Electrophilicity. Note: It polarizes the P-O bonds.

Structural Information


reagent of MG 1156 B
Download the coordinates file
Representative Structure
PDB code 2qf7
MACiE Metal Type
(Identity)
magnesium
(MG1156B)
PDB Metal Type (Identity) magnesium
(MG1156A)
Oxidation State 2
Coordination Geometry
(Number of ligands)
octahedral
(4)

Some water molecules are probably missing in the structure. This explains why the coordination number is less than 6.


First Coordination Sphere


Ligand Type Ligand (PDB) Ligand (MACiE) Catalytic Binding Mode Contacting Atoms
Amino Acid Residue GLU 297 AGLU 297 A No bidentate OE1,OE2
Reactive Species SAP 1162 A- No bidentate O2G,O2B
Note: It is a nonhydrolyzable ATP analog.




Right Arrow
MACiE Metal Type
(Identity)
magnesium
(MG1156B)
Oxidation State 2
Coordination Geometry
(Number of ligands)
octahedral
(6)

This step has no available structures in the PDB: all the coordinating information are derived from the reaction model in MACiE.


Step 02

Metal Functions

Substrate Binding

Structural Information


MACiE Metal Type
(Identity)
magnesium
(MG1156B)
Oxidation State 2
Coordination Geometry
(Number of ligands)
octahedral
(6)

This step has no available structures in the PDB: all the coordinating information are derived from the reaction model in MACiE.





Right Arrow
MACiE Metal Type
(Identity)
magnesium
(MG1156B)
Oxidation State 2
Coordination Geometry
(Number of ligands)
octahedral
(6)

This step has no available structures in the PDB: all the coordinating information are derived from the reaction model in MACiE.


Step 03

Metal Functions

Substrate Binding

Structural Information


MACiE Metal Type
(Identity)
magnesium
(MG1156B)
Oxidation State 2
Coordination Geometry
(Number of ligands)
octahedral
(6)

This step has no available structures in the PDB: all the coordinating information are derived from the reaction model in MACiE.





Right Arrow
MACiE Metal Type
(Identity)
magnesium
(MG1156B)
Oxidation State 2
Coordination Geometry
(Number of ligands)
octahedral
(6)

This step has no available structures in the PDB: all the coordinating information are derived from the reaction model in MACiE.


Step 04

Metal Functions

Substrate Binding

Structural Information


MACiE Metal Type
(Identity)
magnesium
(MG1156B)
Oxidation State 2
Coordination Geometry
(Number of ligands)
octahedral
(6)

This step has no available structures in the PDB: all the coordinating information are derived from the reaction model in MACiE.





Right Arrow
MACiE Metal Type
(Identity)
magnesium
(MG1156B)
Oxidation State 2
Coordination Geometry
(Number of ligands)
octahedral
(6)

This step has no available structures in the PDB: all the coordinating information are derived from the reaction model in MACiE.


Step 05

Metal Functions

Not Present. Note: This step occurs in the carboxyltransferase domain. Mg is bound to the biotin carboxylase domain.

Structural Information


MACiE Metal Type
(Identity)
magnesium
(MG1156B)
Oxidation State 2
Coordination Geometry
(Number of ligands)
unknownGeometry
(9999)

This step occurs in the carboxyltransferase domain. Mg is bound to the biotin carboxylase domain.

This step has no available structures in the PDB: all the coordinating information are derived from the reaction model in MACiE.





Right Arrow
MACiE Metal Type
(Identity)
magnesium
(MG1156B)
Oxidation State 2
Coordination Geometry
(Number of ligands)
unknownGeometry
(9999)

This step occurs in the carboxyltransferase domain. Mg is bound to the biotin carboxylase domain.

This step has no available structures in the PDB: all the coordinating information are derived from the reaction model in MACiE.


Step 06

Metal Functions

Not Present. Note: This step occurs in the carboxyltransferase domain. Mg is bound to the biotin carboxylase domain.

Structural Information


MACiE Metal Type
(Identity)
magnesium
(MG1156B)
Oxidation State 2
Coordination Geometry
(Number of ligands)
unknownGeometry
(9999)

This step occurs in the carboxyltransferase domain. Mg is bound to the biotin carboxylase domain.

This step has no available structures in the PDB: all the coordinating information are derived from the reaction model in MACiE.





Right Arrow
MACiE Metal Type
(Identity)
magnesium
(MG1156B)
Oxidation State 2
Coordination Geometry
(Number of ligands)
unknownGeometry
(9999)

This step occurs in the carboxyltransferase domain. Mg is bound to the biotin carboxylase domain.

This step has no available structures in the PDB: all the coordinating information are derived from the reaction model in MACiE.


Step 07

Metal Functions

Not Present. Note: This step occurs in the carboxyltransferase domain. Mg is bound to the biotin carboxylase domain.

Structural Information


MACiE Metal Type
(Identity)
magnesium
(MG1156B)
Oxidation State 2
Coordination Geometry
(Number of ligands)
unknownGeometry
(9999)

This step occurs in the carboxyltransferase domain. Mg is bound to the biotin carboxylase domain.

This step has no available structures in the PDB: all the coordinating information are derived from the reaction model in MACiE.





Right Arrow
MACiE Metal Type
(Identity)
magnesium
(MG1156B)
Oxidation State 2
Coordination Geometry
(Number of ligands)
unknownGeometry
(9999)

This step occurs in the carboxyltransferase domain. Mg is bound to the biotin carboxylase domain.

This step has no available structures in the PDB: all the coordinating information are derived from the reaction model in MACiE.



spacer
spacer