MG 161 x
Metal-MACiE entry for MG 161 x
Two Mg2+ ions are required for the catalysis. Six catalytic roles were proposed for these metal ions: (i) they induce the conformational changes to bring catalytic residues into the active site, (ii) they orient substrates (iii) they activate ATP for the nucleophilic attack (iv) they lower the pKa of the substrate (v) they stabilize the negative charge developed in the transition state (vi) they facilitate the departure of AMP (PMID11080626).
First Coordination Sphere
Activator. Note: It activates ATP.
Increase Electrophilicity. Note: It increases nucleophilicity of ATP.
Electrostatic Stabiliser. Note: It stabilizes the negative charge of the transition state.