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Catalytic Site Atlas Version 2.2.12
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CSA entry for 1yon
Original Entry
Title:
Oxidoreductase
Compound:
2-dehydropantoate 2-reductase
Mutant:
No
UniProt/Swiss-Prot:
P0A9J4-PANE_ECOLI
EC Class:
1.1.1.169
Other CSA Entries:
Overview of all sites for 1yon
Homologues of 1yon
Entries for UniProt/Swiss-Prot: P0A9J4
Entries for EC: 1.1.1.169
Other Databases:
PDB entry: 1yon
PDBsum entry: 1yon
UniProt/Swiss-Prot: P0A9J4
IntEnz entry: 1.1.1.169
Literature Report:
Introduction:
Ketopantoate reductase (KPR), isolated from Escherichia coli, catalyses the reduction of ketopantoate by NADPH to form pantoate and NADP+. This is the second reaction in the pathway for the biosynthesis of pantothenate (vitamin B5). KPR is a member of the 6-phosphogluconate dehydrogenase superfamily. The reaction proceeds by a sequential ordered bi:bi kinetic mechanism, with NADPH binding first, followed by a conformational change and the binding of ketopantoate. After the reaction NADP+ dissociates first, followed by pantoate.
Mechanism:
The pro-S hydride of NADPH is transferred to the si face of the C2 carbonyl of ketopantoate. The developing alkoxide is protonated by Lys176 to form pantoate.
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Found by:
Literature reference 

ResidueChainNumberUniProt numberFunctional part FunctionTargetDescription
LYSA 176 176Sidechain
Acid/baseSubstrate
ElectrostaticSubstrate
Lys176 hydrogen bonds to the oxygen of the ketopantoate carbonyl, polarising the C=O bond and making it more susceptible to nucleophilic attack. It also transfers a proton to the developing alkoxide during the reaction.
Evidence from paper Evidence concerns Evidence type
PubMed ID 10736170 Current protein Conservation of residue
PubMed ID 11123955 Current protein pH dependence of reaction
PubMed ID 17229734 Current protein Residue is positioned appropriately (ligand position known)
PubMed ID 11123955 Current protein Mutagenesis of residue
Notes:

References:
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